Professor of Botany
Ph.D. (1990) Pennsylvania State University
608-265-5294
spalding@wisc.edu
Seedling growth and development is the biology at the center of most of the research activities in our laboratory. Arabidopsis thaliana and maize are the species we use because of the genetic element they allow to augment our cellular, physiological, and developmental studies. Increasing measurement throughput is key to leveraging the genetic resources built up around these model systems. For this reason, we focus on developing the computational tools required to make automated image-based studies of plant growth and development. More about specific current projects and the people working on them can be found by following the links below.
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ABC proteins & auxin |
photomorphogenesis |
glutamate receptors |
See the Science Nation video and the Popular Science article about our Phytomorph project.
Representative Recent Publications (full publication list) Edgar's c.v. & personal page
Spalding EP, Harper JF (2011) The ins and outs of cellular Ca2+ transport. Current Opinion in Plant Biology 14: 715-720
Elwell AL, Gronwall DS, Miller ND, Spalding EP, Durham Brooks TL (2011) Separating parental environment from seed-size effects on next generation growth and development in Arabidopsis. Plant Cell & Environment 34: 291-301
Wu G, Otegui MS, Spalding EP (2010) The ER-localized TWD1 immunophilin is necessary for localization of multidrug resistance-like proteins required for polar auxin transport in Arabidopsis roots. The Plant Cell 22: 3295-3304
Miller ND, Durham Brooks TL, Assadi AH, and Spalding EP (2010) Detection of a gravitropism phenotype in glutamate receptor-like 3.3 mutants of Arabidopsis thaliana using machine vision and computation. Genetics 186: 585-593 selected for Issue Highlights
Spalding EP (2010) The inside view on plant growth. Nature Methods 7: 506-507
Wu G, Cameron JN, Ljung K, Spalding EP (2010) A role for ABCB19-mediated polar auxin transport in seedling photomorphogenesis mediated by cryptochrome 1 and phytochrome B. The Plant Journal 62: 179-191
Durham Brooks TL, Miller ND, Spalding EP (2010) Plasticity of Arabidopsis root gravitropism throughout a multi-dimensional condition space quantified by automated image analysis. Plant Physiology 152: 206-216
Lewis DR, Wu G, Ljung K, Spalding EP (2009) Auxin transport into cotyledons and cotyledon growth depend similarly on the ABCB19 Multidrug Resistance-like Transporter. The Plant Journal 60: 91-101
Wang L, Uilecan IV, Assadi AH, Kozmik CA, Spalding EP (2009) HYPOTrace image analysis software for measuring hypocotyl growth and shape demonstrated on Arabidopsis seedlings undergoing photomorphogenesis. Plant Physiology 149: 1632-1637
Stephens NR, Qi Z, Spalding EP (2008) Glutamate receptor subtypes evidenced by differences in desensitization and dependence on the GLR3.3 and GLR3.4 genes. Plant Physiology 146: 529-538
Wu G, Spalding EP (2007) Separate functions for nuclear and cytoplasmic cryptochrome 1 during photomorphogenesis of Arabidopsis seedlings. Proceedings of the National Academy of Sciences USA 104: 18813-18818
Miller ND, Parks BM, Spalding EP (2007) Computer-vision analysis of seedling responses to light and gravity. The Plant Journal 52: 374-381
Wu G, Lewis DR, Spalding EP (2007) Mutations in Arabidopsis Multidrug Resistance-like ABC transporters separate the roles of acropetal and basipetal auxin transport in lateral root development. The Plant Cell 19: 1826-1837
Lewis DR, Miller ND, Splitt BL, Wu G, Spalding EP (2007) Separating the roles of acropetal and basipetal auxin transport on gravitropism with mutations in two Arabidopsis Multidrug Resistance-like ABC transporter genes. The Plant Cell 19: 1838-1850