Olympus BX60 Epifluorescence Microscope

The NIC has a conventional epifluroescence microscope made by Olympus. It features three cubes (UV, blue and green excitation - see specifications below), two types of arc lamps (mercury or xenon), a sutter excitation filter wheel with shutter, and a digital camera (a color Olympus DP70). This microscope is useful for fluorescence imaging of larger samples (the lowest power objective is a 2.5x) and for screening for fluorescence. Excellent bright field or DIC digital images are also possible to capture using this equipment.

Epifluorescence Mircoscope

 

Filter Cubes, NIC Olympus Epifluorescence Microscope

Position "WU"
U-MWU
Olympus UV excitation
ex 330-385
bs 400
em 420 LP

Position "A"
41020 HQ NB GFP C-53502
Chroma Narrow-band GFP, for minimizing autofluorescence.
ex 480/20
bs 495
em 510/20

Position "WIG"
U-MWIG
Olympus Rhodamine
ex 520-550
bs 565
em 580

Position "NIB"
U-MNIB
Olympus FITC
ex 470-490
bs 505
em 515 LP

 

Two extra cubes not installed:
U-M 61002 403 "DAPI/FITC/TXSRED"
Fura2 (NOTE: NO EX FILTER! Use with filter wheel.) Chroma 71000A C71135

Olympus cube specifications.