Plastid Biogenesis

Plastids are transformed from relatively undifferentiated proplastids to a variety of differentiated forms (chloroplasts, chromoplasts, amyloplasts, etc.) through changes in protein composition and membrane organization. This is mediated through translocation systems that ensure the proteins are directed to the right place at the right time. We have been studying the internal targeting systems related to bacterial targeting systems. Mutations in components of the Sec systems can block plastid biogenesis at very early stages (in embryos) or block the transition to chloroplasts at later stages (in older embryos and seedlings). We have characterized mutants and generated lines carrying tagged proteins to aid in our continuing studies of the function of these systems.

Supported by the UW-Graduate School

See:

Martin, J.R., J. H. Harwood, M.W. McCaffery, D.E. Fernandez, K.C. Cline (2009). Localization and integration of thylakoid protein translocase subunit cpTatC. Plant J. 58: 831-842.

Skalitzky, C.A., J.R. Martin. J.H. Harwood, J.J. Beirne, B.J. Adamczyk, G.R. Heck, K. Cline, and D.E. Fernandez (2011).  Plastids contain a second Sec translocase system with essential functions.  Plant Physiol.  155: 354-369.